ABSTRACT
CD4+CD25+ regulatory T cells (CD4+CD25+ Tregs) have been shown to play a regulatory or suppressive role in the immune response and are possibly relevant to the pathogenesis of autoimmune diseases. In the present study, we attempted to investigate the frequency of CD4+CD25+ Tregs in peripheral blood (PB) of collagen-induced arthritis (CIA) rats during the development of arthritis, to determine whether their frequency is involved in the immunoregulation of this disease. The results showed that normal rats had similar frequencies of CD4+CD25+ Tregs in PB during the experiment time, expressed as a percentage of CD4+CD25+Foxp3+ T cells among the CD4+ T lymphocyte population. In contrast, the frequency of CD4+CD25+Foxp3+ T cells in CIA rats was found to change during the development of arthritis. In CIA rats, there is a significant negative correlation between the frequency of CD4+CD25+Foxp3+ T cells and paw swelling (r=-0.786, p< 0.01). The relationship between the frequency of CD4+CD25+Foxp3+ T and immune activation was not found in normal rats. During the time course, the frequency of CD4+CD25+Foxp3+ T was lower in CIA rats than in normal ones. The data suggest that the frequency of PB CD4+CD25+ Tregs may be a promising marker for arthritis activity.
Subject(s)
Animals , Rats , Arthritis , Arthritis, Experimental , Autoimmune Diseases , Lymphocytes , T-Lymphocytes , T-Lymphocytes, RegulatoryABSTRACT
Objective To analyze the usage of antibiotics after renovation in the use of this present situation and existing problems of pediatrics.Methods By application of our self-developed analyzing system for the clinical use of antibacterial agents,the prescriptions of pediatric outpatient in 2012 was studied retrospectively and their rationality was evaluated.Results The antibiotic prescription rate was 37.7％ in pediatric outpatient and emergency in our hospital,their were 26 antibacterial agents in clinic use which were involved in 6 pharmacological group.The defined daily dose(DDD) analysis was adopted and the results have shown that macrolides (42.20％),cephalosporins (35.90％) and penicillins (20.90％) were the most prescribed antibiotics and most of them were oral medicines (72.2％) and non restrictive drug (61.0％).The antibacterial agents' s combination therapy rate was 2.4％.Irrational use of drugs mainly include the wrong way of administration (78.0％),application of antibiotics with no indications (21.3％) and irrational combined use of antibiotics (0.5％).Conclusion The application of antibiotics in our hospital pediatric basic structure reasonable,but there are still improper usage and dosage,no indication of medicine,etc.It is necessary to further improve and perfect the use and management of antimicrobial agents.
ABSTRACT
OBJECTIVE: To evaluate the in vitro MTT assay for determining the drug sensitivity in fresh human tu- mors. METHODS: We tested the samples from 75 patients with gastric cancer, 55 patients with colorectal carcinoma, 55 patients with bladder cancer and 6 patients with carcinoma of tongue. RESULTS: On the average, the MTT sensitive drug in the above - mentioned first three kinds of solid tumors corresponded with the drug used by clinican experience. The response rate in MTT sensitive group was 73. 8% (48/65) while only 34.0% (17/50) in the control group and 0% (0/10) in MTT resistant group. CONCLUSION: MTT assay appears to be useful in exploring the possible anticancer effect of arsenic trioxide on tongue cancer. This test demonstrats that the MTT assay is clinically useful on an individual basis to optimize chemotherapy.
ABSTRACT
OBJECTIVE:To facilitate the evolvement of hospital pharmaceutical care.METHODS:The website of pharmacy department of Shanghai No.9People’s Hospital was programmed and exploited,and based on which the hospital pharmaceutical information service was carrying out.RESULTS:The website ran stably and fast;its maintenance was very convenient;pharmaceutical information was rich,the online communication between doctors and patents was available here and the application outcome proved to be satisfactory.CONCLUSION:Establishing hospital pharmaceutical website and carrying out online pharmaceutical care are of significance in the evolvement of hospital pharmacy work.